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Literature summary for 2.6.1.16 extracted from

  • Li, P.; Li, K.; Li, X.; Zhao, F.; Wang, R.; Wang, J.
    Improving enzyme activity of glucosamine-6-phosphate synthase by semi-rational design strategy and computer analysis (2020), Biotechnol. Lett., 42, 2319-2332 .
    View publication on PubMed
Search for more literature for 2.6.1.16

Cloned(Commentary)

Cloned (Comment) Organism
gene glmS, screening of mutant library, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
A594G site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) Bacillus subtilis
K595H site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) Bacillus subtilis
L593S site-directed mutagenesis, the mutant shows highly increased activity (48 U/l) compared to wild-type (5 U/l) Bacillus subtilis
additional information improvement of enzyme BsGlms activity through computer simulation and site saturation mutagenesis, method, overview Bacillus subtilis
S596F site-directed mutagenesis, the mutant shows slightly increased activity (7 U/l) compared to wild-type (5 U/l) Bacillus subtilis
V597R site-directed mutagenesis, the mutant shows slightly increased activity (8 U/l) compared to wild-type (5 U/l) Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information binding energy calculations of wild-type and mutant enzymes Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + D-fructose 6-phosphate Bacillus subtilis
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?
L-glutamine + D-fructose 6-phosphate Bacillus subtilis 168
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P0CI73
-
-
Bacillus subtilis 168 P0CI73
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis L-glutamate + D-glucosamine 6-phosphate
-
 ?
L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis 168 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
BsglmS
-
 Bacillus subtilis
GlmS
-
 Bacillus subtilis
glucosamine-6-phosphate synthase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bacillus subtilis

General Information

General Information Comment Organism
additional information mutation of residue Ser593 enhances the flexibility of the protein, which ultimately leads to increased enzyme activity, molecular dynamics simulation, homology modeling, and protein-ligand docking, overview Bacillus subtilis